电离辐射通过转化生长因子-β-介导的上皮-粘液转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨强磁场是否可通过转化内皮线粒体-β（TGF-β）-激活的粘液-糖蛋白转换 (EMT)来作出贡献免疫线粒体的洪水泛滥迁移。使用增幅2Gy(60)Coγ线太阳光源自人类所循环系统的6种免疫线粒体，记录与EMT相关的发生变化，这包括分别运用显微镜电子技术，酶质区别于方国法，免疫荧光电子技术，划痕检验和Transwell小室检验来推论并验证线粒体三组织形态，EMT标有，洪水泛滥迁移能力等。转用酶联免疫吸附国法验证这些免疫线粒体之前TGF-β酶水平，运用引人注意类固醇SB431542来评估TGF-β瞬时路中在强磁场EMT之前的作用。经过增幅为2Gy太阳光的免疫线粒体之前存在间叶线粒体的传达，与假太阳光三组相比其粘液标有减小，间叶线粒体标有增加，同时其洪水泛滥转回能力增强，TGF-β酶水平也提升。进一步发现由A549强磁场诱导的EMT可通过对TGF-β瞬时抑制发生扭转。这些结果表明TGF-β激活的EMT在强磁场诱导增强免疫线粒体洪水泛滥转回能力之前起着不可忽视。

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